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MALDI-TOF analysis of DCCD labeling of the F1Fo-ATP synthase from strain TA2.A1. DCCD labeling was performed with 20 μM DCCD in 50 mM potassium phosphate buffer (pH 7.5) for 20 min and was stopped by addition of 10 volumes of chloroform-methanol (1:1). Isolation of subunit c and sample preparation for MALDI-TOF MS were performed as described previously (45).
