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Northern analysis of gadB and gadC transcription in E. coli MC4100 and isogenic hns, rpoS, and hns rpoS mutants. Total RNA was extracted from cultures of each strain at the growth curve points of A600 of 1.0, 1.5, and 1.8. The membrane was probed with the 32P-labeled internal fragments of gadB (A) and gadC (B).
