FIG. 2.

Transcriptional analysis of cwp cluster. Hybridization DNA-membrane-immobilized RNA on strain 630; 15, 30, and 25 μg of total RNA were used for hybridization with probes specific to slpA (A), cwp66 (B), and cwp84 (C), respectively. MW, RNA molecular weight marker (Sigma); lanes 1A, 1B, and 1C, RNA from stationary growth phase (18-h culture); lanes 2A, 2B, and 2C, RNA from the middle of the exponential growth phase (OD₆₀₀ of ∼0.7); lanes 3A, 3B, and 3C: RNA from the beginning of the exponential growth phase (OD₆₀₀ of ∼0.3). Sizes of transcripts are indicated with arrows. The probe specific for the transcript of the gdh gene hybridized with an estimated 1.3-kb transcript in the sample corresponding to the different growth phase, as expected (16) (data not shown).