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. 2003 Aug;185(15):4461–4470. doi: 10.1128/JB.185.15.4461-4470.2003

FIG. 4.

FIG. 4.

RT-PCR analysis of the genes located upstream from slpA (A) and cwp66 (B) in strain 630. (A) MW, molecular weight marker (100-bp ladder; Amersham Biosciences). Lanes 1 to 3, amplification between orf6 and slpA with orf6-RT1/slpA-RTR1 primers, demonstrating the presence of an intergenic mRNA. Lanes 4 to 6, no amplification was obtained with the primers slpA-RT1/orf7-RTR1, showing absence of intergenic RNA messenger. RNA was extracted from the stationary phase (lanes 1 and 4), middle exponential growth phase (lanes 2 and 5), and beginning of the exponential growth phase (lanes 3 and 6). (B) MW, molecular weight marker (100 bp ladder). Lanes 1 to 3, RNA extracted from the beginning of the exponential growth phase. Lane 1, cwp66-NB1/cwp66NBR1 primers; lane 2, orf8-RT1/orf9-RTR1 primers; lane 3, orf9-RT1/cwp66-RTR1 primers.