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. 2000 Jun 20;97(13):7509–7514. doi: 10.1073/pnas.97.13.7509

Figure 2.

Figure 2

Western blot analysis of EBA-175 protein expression. (A) Western blot analysis (5% SDS/PAGE) of parasite pellet and culture supernatant proteins isolated from W2-mef and the clones W2-m/Δc.1 and W2-m/Δc.2. The membrane was probed with antibodies directed toward regions III–V of EBA-175 and indicates that the approximately 160-kDa truncated form of EBA-175 is expressed and released into the culture supernatant at similar levels to the wild-type protein. Before its release into the culture supernatant, wild-type EBA-175 has an approximate molecular weight of 190 kDa. The soluble form of EBA-175 present within culture supernatant has an apparent molecular weight of 175 kDa. (B) Western blot analysis of parasite pellet proteins probed with antibodies raised against the 3′cys-rich domain confirms the absence of this region within the truncation mutants. The arrow indicates the position of wild-type EBA-175 detected with these antibodies.