Figure 3.

Immunolocalization analysis of wild-type and mutant EBA-175. Representative images of indirect immunofluorescence labeling using antibodies directed toward regions III–V of EBA-175 are presented for W2-mef and W2-m/Δc.1 parasites. In each case, staining was most intense in the apical region of developing merozoites within segmenting schizonts and also free merozoites released after schizont rupture. This punctate fluorescence pattern is characteristic of antigens localized within the apical complex of merozoites.