TABLE 1.
GFP fluorescence intensity in strain B13 (Pint-gfp) grown in batch culturea
| Incubation time (h) | Fructose
|
CBA
|
||
|---|---|---|---|---|
| GFP fluorescence intensity (AGV units) | Top 5% MFV | GFP fluorescence intensity (AGV units) | Top 5% MFV | |
| 14b | 79.6 (79.5-79.6) | 80.4 | 79.6 (79.5-79.7) | 80.3 |
| 40 | 81.8 (81.6-82.0) | 86.8 | 87.43 (86.2-88.4) | 94.9 |
| 64 | 84.0 (83.5-84.8) | 92.4 | 96.5 (94.9-97.9) | 104.4 |
| 88 | 83.3 (82.2-84.1) | 91.7 | 94.0 (93.1-94.9) | 102.5 |
a
Fructose and 3-chlorobenzoate were used at 10 mM. GFP fluorescence intensity is given as cellular average gray value units of the 95th percentile of the population. Values in parentheses represent the 95% confidence intervals, calculated with the R software. MFV values are the arithmetic means of the cellular AGVs for the 5% of the population with the highest fluorescence intensities.
b
At 14 h, both fructose- and 3-chlorobenzoate-grown cultures were growing exponentially and entered the stationary phase after about 24 h of incubation.