TABLE 3.
GFP production in strain B13 (Pint-gfp) at different cell densitiesa
| Cell culture | GFP fluorescence intensity (AGV units) with 0.1 mM CBA
|
GFP fluorescence intensity (AGV units) with 10 mM CBA
|
||
|---|---|---|---|---|
| 95th percentile | Top 5% MFV | 95th percentile | Top 5% MFV | |
| Chemostat, 6 days | 83.8 (83.7-84.2) | 86.0 | 80.9 (80.8-81.0) | 84.7 |
| Chemostat, 14 days | 83.8 (83.5-84.1) | 88.8 | 81.0 (80.8-81.2) | 82.5 |
| Starvation, 60 h | 85.1 (83.8-87.4) | 94.5 | 85.3 (84.2-86.3) | 93.3 |
Cells were taken from the chemostat after 6 days or 14 days of continuous cultivation or taken on day 6 and then incubated in batch culture without any carbon for 60 h. Cells were grown with 0.1 mM (OD600 = 0.01) or 10 mM (OD600 = 0.83) CBA. GFP fluorescence intensity values are given as for Table 1. Note that absolute values of GFP fluorescence in this table cannot be compared to those in Table 2 due to slight variations in the brightness of the UV light source.