TABLE 5.
Transfer of c/c element on membrane filters in the presence of CBA or fructosea
| Mating time (h) | Mean transfer frequency (SD)
|
Incubation period (h) | Transfer rate
|
||
|---|---|---|---|---|---|
| CBA | Fructose | CBA | Fructose | ||
| 24 | 1.27 × 10−3 (0.21 × 10−3) | 0.63 × 10−3 (0.34 × 10−3) | 0-24 | 5.28 × 10−5 | 2.61 × 10−5 |
| 48 | 50.8 × 10−3 (11 × 10−3) | 9.05 × 10−3 (3.0 × 10−3) | 24-48 | 2.06 × 10−3 | 0.35 × 10−3 |
| 72 | 249 × 10−3 (84 × 10−3) | 89.8 × 10−3 (42 × 10−3) | 48-72 | 8.27 × 10−3 | 3.36 × 10−3 |
a
Both CBA and fructose were present at 1 mM in the filter support medium. The mean transfer frequency (standard deviation) was calculated as the number of transconjugants per donor cell counted after 24, 48, and 72 h of mating time. The transfer rate was calculated as the net increase in the number of transconjugants per donor cell per hour during the corresponding incubation period.