FIG. 3.
Kinetics of changes in gene expression during the response of C. glutamicum ATCC 13032 to Pi starvation. Changes in gene expression 10, 30, 60, 90, 120, and 180 min after a shift from Pi-sufficient to Pi-limiting conditions compared to preshift conditions taken from Table 2 are shown for selected ORFs (see text for details). (A to D) Kinetics of changes in gene expression for subcluster 1 genes (Fig. 2): genes encoding the putative high-affinity phosphate transporter pstSCAB (ORFs 2871 to 2876) (A), the glycerophosphoryl diester phosphodiesterase and the sn-glycerol 3-phosphate transport system (glpQ and ugpAEBC, ORFs 2637 to 2641) (B), and the putative extracellular nuclease (ORF 2851, nucH), 5′-nucleotidase or a related esterase (Cgl0328, ORF 766), and the hypothetical proteins (ORFs 1086, 3081, and 3082) (C) and all other subcluster 1 genes (D). (E and F) Kinetics of changes in gene expression for the subcluster 2 genes (Fig. 2) except ORF 2831 (E) and for subcluster 3 genes and ORF 2831 (F).