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. 2003 Aug;185(15):4519–4529. doi: 10.1128/JB.185.15.4519-4529.2003

TABLE 2.

Changes in gene expression at different times during the Pi starvation response of C. glutamicum

ORF NCBI no. Annotation Gene Avg mRNA level after shift of Pi-limiting conditions/avg mRNA level before shift to Pi-limiting conditionsb
10 min 30 min 60 min 90 min 120 min 180 min
195 Cgl2814 Sulfate adenylate transferase subunit 1 2.2c 0.3c 0.2c 0.4c
312 Cgl2917 Putative integral membrane transport protein, similar to shikimate transport protein shiA 10.0 0.4 0.6 0.5c
348 Cgl2946 Hypothetical membrane protein 1.2c 0.7 0.3c 0.2c 0.2c 0.1c
376 Similar to heavy-metal-transporting ATPases 2.2c 5.7c 3.4 2.5 1.3 1.0
378 Cgl2965 Two-component system, response regulator 1.5 7.1c 6.0c 4.3c 2.1c 1.3
380 Cgl2966 Hypothetical protein 8.3c 6.4c 2.7c 1.3 1.0
381 Cgl2967 Putative multicopper oxidase cumA 1.8c 9.3c 6.1c 3.1c 1.3 0.8
383 Cgl2968 Hypothetical protein 1.8 5.7c 3.4 1.0 0.6c
399 Cgl2979 Hypothetical protein 3.9c 4.8c 1.7 1.1 1.1 0.9
403 Cgl2982 Single-stranded DNA-binding protein ssb 0.6c 0.2c 0.2c
441 Cgl0065 Phosphate starvation-inducible protein phoH 1.1 1.8 3.3c 4.4c 4.7c 4.5c
442 Cgl0065 Phosphate starvation-inducible protein phoH 1.4 1.4 5.9c 4.3c 6.8c 4.8c
766 Cgl0328 5′-Nucleotidase or related esterase 1.2 7.3c 6.5c 19.3c 10.3c 7.2c
854 Cgl0388 Hypothetical membrane protein 0.2c 0.1c 0.2c 0.3c 1.9 1.3
856 Cgl0390 ABC-type transporter, permease component, similar to heme uptake systems 0.4c 0.2c 0.3 0.5 1.6
857 Cgl0391 ABC-type transporter, ATPase component, similar to heme uptake systems 0.3c 0.2c 0.3 0.5 1.5 0.9
976 Cgl0485 Ribosomal protein L10 0.8 0.8 0.2c 0.1c 0.1c 0.0c
978 Cgl0486 Ribosomal protein L7/L12 0.7 0.8 0.2c 0.3c 0.3 0.2c
988 Cgl0493 Ribosomal protein S12 1.5c 0.8 0.3c 0.3c 0.2c 0.2c
992 Cgl0514 Ribosomal protein S3 0.9 0.7 0.2c 0.1c 0.1c 0.1c
993 Cgl0515 Ribosomal protein L16/L10E 0.7 0.1c 0.1c
994 Cgl0516 Ribosomal protein L29 0.9 0.7 0.2c 0.1c
999 Cgl0521 Ribosomal protein L14 0.3c 0.2c
1000 Cgl0522 Ribosomal protein L24 1.2 0.8 0.2c 0.2c 0.1c 0.1c
1020 Cgl0539 Ribosomal protein L18 1.0 0.7 0.2c 0.2 0.2 0.1c
1044 Cgl0560 Translation initiation factor IF-1 1.1 0.4 0.3c 0.4 0.2c 0.4
1047 Cgl0562 Ribosomal protein S11 1.1 0.7 0.2c 0.2c 0.2c
1048 Cgl0563 Ribosomal protein S4 1.0 0.8 0.3c 0.2c 0.2c
1050 Cgl0566 Ribosomal protein L17 1.0 0.7 0.3c 0.2c 0.2c
1086 Cgl0596 Hypothetical protein 10.8c 18.8c 17.6c 15.0c 8.9c
1168 Cgl0665 Similar to vibriobactin utilization protein viuB 0.1c 0.3c 1.3 0.8
1169 Cgl0666 ABC-type transporter, ATPase component, similar to iron uptake systems 0.2c 0.2c 1.1 0.7
1170 Cgl0667 ABC-type transporter, permease component, similar to iron uptake systems 0.1c 0.2c 1.0
1173 Cgl0669 ABC-type transporter, periplasmic component, similar to iron uptake systems 0.2c 0.2c 0.2c 0.3c 1.0 0.7
1205 Cgl0695 Methylisocitrate lyase prpB1 3.3c 3.5c 4.7c 3.9c
1206 Cgl0696 Methylcitrate synthase prpC1 0.6 1.2 3.7c 4.2c 2.9c
1346 Cgl0807 Siderophore-interacting protein 0.1c 0.2c 1.1
1347 Cgl0808 ABC-type cobalamin/Fe3+-siderophore transport 0.1c 0.1c 0.1c 0.2c 1.3 0.8
1349 Cgl0810 ABC-type cobalamin/Fe3+-siderophore transport 0.1c 0.1c 0.2c 0.2c 1.0 0.5
1351 Cgl0812 ABC-type cobalamin/Fe3+-siderophore transport 0.2c 0.2c 0.3c
1352 Cgl0813 ABC-type cobalamin/Fe3+-siderophore transport 0.1c
1420 Similar to ribosomal protein L33 of E. coli (putative sequencing error) 1.1 0.6 0.2c 0.4 0.2c
1421 Cgl0869 Ribosomal protein L28 1.0 0.8 0.2c 0.2c 0.2c 0.2c
1558 Cgl0982 AraC-type DNA-binding domain-containing protein 0.2c 0.2c 0.2c 0.4c 2.6 1.1
1598 Cgl1021 Hypothetical membrane protein 0.8 1.6c 4.5c 7.8c 7.8c 6.9c
1645 Cgl1067 Cysteine sulfinate desulfinase 1.1c 1.2 0.5c 0.3c 0.2c 0.3c
1646 Cgl1068 Nicotinate-nucleotide pyrophosphorylase 1.0 1.0 0.3c 0.1c 0.1c 0.1c
1647 Cgl1069 Quinolinate synthase 0.8 1.1 0.3c 0.1c 0.1c 0.1c
1655 Cgl1077 2-Polyprenyl-6-methoxyphenol hydroxylase 4.6c 1.6 0.9c 1.0 1.0 0.9
1722 Cgl1139 Methionine synthase II 0.7 0.2c 0.2c 0.6c
1855 Cgl1248 Siderophore-interacting protein 0.4c 0.3c 4.3
1875 Cgl2996 myo-Inositol-1-phosphate synthase 0.8c 1.3c 0.8 0.4c 0.2c 0.5c
2146 Cgl2035 ABC-type transport systems, periplasmic protein, similar to iron(III) dicitrate uptake systems 0.3c 0.2c 0.3c 1.6 1.0
2168 Questionable ORF 1.6c 1.1 0.3c 0.2c 0.2c
2261 Cgl2133 Predicted Co/Zn/CD cation transporter 0.8 1.2 2.0c 2.7c 3.5c 4.8c
2262 Cgl2134 Dehydrogenase 0.6c 1.2 2.3c 2.5c 3.3c 4.4c
2553 Cgl1460 ABC-type transporter, ATPase component 1.0 2.8 3.0c 5.0c 2.7c 2.3/PICK>
2554 Cgl1459 ABC-type transporter, permease component 1.2 6.3 3.6c 3.4c 2.9c 2.1
2614 Questionable ORF 1.2c 0.7 0.3c 0.2c 0.2c 0.1c
2624 Cgl1399 Arginine repressor argR 5.0c 0.7 0.7c 0.8 0.8 0.8
2625 Cgl1398 Ornithine carbamoyltransferase argF 5.5c 0.6 0.6c 0.6c 0.7 0.7
2637 Cgl1387 Glycerophosphoryl diester phosphodiesterase glpQ 1.5 2.8c 4.7c 5.0c 9.2c 7.5c
2638 Cgl1386 Glycerol-3-phosphate ABC-type transporter, ATPase component ugpC 1.1 4.2c 3.9c 4.3c 4.5c 4.5c
2639 Cgl1385 Glycerol-3-phosphate ABC-type transporter, periplasmic component ugpB 2.2 12.9c 22.8c 18.7c 17.2c 14.5c
2640 Cgl1384 Glycerol-3-phosphate ABC-type transporter, permease component ugpE 1.6c 9.8c 22.3c 15.0c 11.7c 18.1c
2641 Cgl1383 Glycerol-3-phosphate ABC-type transporter, permease component ugpA 2.1 15.1c 16.8c 15.0c 11.0c 12.5c
2647 Cgl1379 Ribosomal protein L35 1.6c 1.0 0.4c 0.3c 0.2c 0.2c
2648 Cgl1378 Translation initiation factor IF3 2.0c 1.1 0.3c 0.2c 0.1c 0.2c
2779 Cgl1281 Co/Zn/Cd efflux system component 3.8c 4.7c 1.9c 1.0 0.6c 0.7c
2831 Cgl2607 Two-component system, response regulator 8.1c 2.3 1.4 1.1 1.1 1.5c
2832 Cgl2606 Two-component system, sensory transduction 4.7c 1.7 1.0 0.8 0.8 0.9
2849 Cgl2594 Predicted deacetylase 4.7 2.0c 2.1 2.1c
2851 Cgl2592 Predicted extracellular nuclease nucH 4.8c 19.5c 31.7c 25.8c 16.9c
2871 Cgl2575 Phosphate ABC-type transporter, periplasmic component pstS 5.0c 18.0c 23.2c 21.5c 19.1c 13.8c
2873 Cgl2574 Phosphate ABC-type transporter, permease component pstC 4.4c 19.3c 23.6c 22.8c 20.9c 19.1c
2875 Cgl2573 Phosphate ABC-type transporter, permease component pstA 1.9 5.8c 12.5c 8.6 7.1c 6.9c
2876 Cgl2572 Phosphate ABC-type transporter, ATPase component pstB 2.7c 9.0c 12.4c 14.5 10.3c 8.3c
2895 Questionable ORF 1.3 0.7 0.3c 0.2c 0.2c 0.1c
3081 Cgl2336 Hypothetical protein 1.0 2.8c 3.8c 4.2c 4.6c 4.2c
3082 Cgl2336 Hypothetical protein 4.3c 5.3 5.0c 5.3c
3086 Questionable ORF 0.5c 0.2 0.4c 0.3c 0.5c
3326 Cgl1067 Cysteine sulfinate desulfinase 0.6 0.2c
3347 Cgl1714 Hypothetical protein 0.2c 0.2 0.3c 4.2 2.5
3362 Questionable ORF 1.8c 2.7 4.7c
3442 Cgl3064 Hypothetical membrane protein, similar to phosphoesterases 2.3 5.3 7.3 11.9c 6.3
3458 Cgl3075 ABC-type transport systems, periplasmic component, similar to iron uptake systems 0.1c 0.1c 0.1c 0.1c 0.5 0.3
3472 Cgl2526 Ferritin-like protein 10.3c 10.3c 5.8c 5.5c 1.8 1.6
64185 Cgl2389 Acyl-CoA:acetate CoA transferase beta subunit 7.3c 1.5 0.4
64187 Cgl2393 Predicted hydrolase/acyltransferase 7.7c 1.7 0.8
66073 Cgl2397 Protocatechuate 3,4-dioxygenase beta subunit 5.2c 1.8 1.3 1.1 1.1 1.0
66513 Cgl2392 Acetyl-CoA acetyltransferase 4.4c 1.8 0.6 0.5 0.7
69134 Cgl2642 Phosphotransferase system IIC component 1.3 0.5 0.4c 0.2c 0.3c 0.2
a

The relative mRNA levels 10, 30, 60, 90, 120, and 180 min after the shift to Pi-limiting conditions compared to the levels under preshift conditions are averages from three experiments. Only ORFs whose mRNA levels were equal to or greater than 4 or equal to or less than 0.25 at at least one time are shown.

b

Boldface type indicates relativemRNA levels that were equal to or greater than 10.

c

P < 0.05 as determined by a t test.