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Heme-linked peroxidase staining of periplasmic fractions from S. frigidimarina PSD106 (ifcR) and PSD201 (ifcR+) after anaerobic growth with ferric citrate. Aliquots (40 μg) of periplasmic fractions were loaded onto an SDS-10% (wt/vol) PAGE gel. Lane 1, pure IfcA protein (1 μg); lane 2, periplasm from S. frigidimarina PSD106 (ifcR); lane 3, periplasm from S. frigidimarina PSD201 (PSD106 complemented with ifcR+); lane 4, purified Fcc3 protein (1 μg).
