TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | BRLa |
| S17-1 | thi pro hsdR hdsM+recA, chromosomal insertion of RP4-2(Tc::Mu Km::T7) | 29 |
| S. frigidimarina strains | ||
| NCIMB400R | Wild type | |
| PSD1 | NCBIMB400R ifcA::ΩCmr | 5 |
| PSD106 | NCBIMB400R ifcR::ΩCmr | This study |
| PSD201 | Complemented PSD106 carrying pPD1 Cmr, Tcr | This study |
| PSR33 | Complemented PSD106 carrying pFR33 Cmr, Tcr | This study |
| Plasmids | ||
| pBluescript II KS+ | Cloning vector, Ampr | Stratagene |
| pJQ200KS | Mobilizable suicide vector, Gmr | 26 |
| pRK415 | IncP broad-host-range cloning vector, Tcr | 13 |
| pHP45ΩCm | Cloning vector containing ΩCm, Ampr | 6 |
| pTRN1 | 2-kb XbaI-XhoI PCR fragment carrying ifcR cloned into pBluescript II KS+ | This study |
| pTRN2 | 4-kb EcoRI fragment containing ΩCm cloned into pTRN1 | This study |
| pTRN3 | 6-kb XbaI-ApaI fragment containing ifcR::ΩCmr cloned into pJQ200KS | This study |
| pTCM1 | 6-kb XbaI fragment carrying ifcR, ifcO, and ifcA::ΩCmr cloned into pBluescript II KS+ | This study |
| pPD1 | 1.3-kb XbaI-PstI fragment carrying ifcR plus 5′ and 3′ flanking sequences cloned into pRK415 | This study |
| pFR3 | 890-bp fragment carrying ifcR cloned into expression vector pT7-7 | This study |
| pFR33 | 932-bp fragment carrying a promotorless ifcR cloned into pRK415 | This study |
a
BRL, Bethesda Research Laboratories.