TABLE 1.
Bacterial strains, primers, vectors, and plasmids used
| Strain, plasmid, or primer | Genotype, sequence (position), and/or comment(s) | Source or reference |
|---|---|---|
| Strains | ||
| D. vulgaris subsp. vulgaris Hildenborough | ||
| Wild type | NCIMB 8303; isolated from clay soil near Hildenborough, United Kingdom | 19 |
| Hyd100 | ΔhydA,B; Cmr | 18 |
| ADH100 | Δadh; Cmr | This study |
| ADH8 | pNotΔAdhCmMob integrated into the chromosome; Sucs Cmr | This study |
| E. coli S17-1 | thi pro hsdR hsdM+ recA RP4-2 (Tc::Mu, Km::Tn7) | 25 |
| Plasmids | ||
| pUC19Cm | pUC19 containing the cat gene | 7 |
| pNOT19 | Cloning vector pUC19; NdeI site replaced by a NotI site | 24 |
| pMOB2 | Contains oriT of plasmid RP4 and Bacillus subtilis sacBR genes on a 4.5-kb NotI fragment; Kmr Cmr | 24 |
| pNotAdh, pNotΔAdh, pNotΔAdhBam, pNotΔAdhCmMob | This study (see text) | |
| Primers | ||
| P206-f | 5′-cccggtaCCATGCTTCAGGGGCTGAAGCCC (−581 to −560)a | |
| P207-r | 5′-ACAACTGGAGCAGGACAGGC (1627 to 1647)a |
a
Positions relative to the adh coding region (bp 1 to 1179). Lowercase letters in P206-f are bases added to create a KpnI cleavage site.