TABLE 3.

Relative expression level (I_R) of the most highly expressed genes in a set of 145 represented on the macroarray^a

ORFb	Gene	Description	IRc	Positiond
2977	adh	Alcohol dehydrogenase	14.8 ± 0.9	5, 7
3581	dsrC	Dissimilatory sulfite reductase	12.9 ± 2.8	6, 4
4250	cyc	Cytochrome c3	12.3 ± 0.8	7, 14
336, 338	apsBA	APS reductase	11.0 ± 0.4	1, 13
5313, 5314, 5316	dsrABD	Dissimilatory sulfite reductase	5.9 ± 0.5	9, 4
219	atpD	ATP synthase F1 β-subunit	5.1 ± 0.5	1, 6
1081	sat	Sulfate adenylyltransferase	4.8 ± 0.2	2, 12
3986	por	Pyruvate:ferredoxin oxidoreductase	3.5 ± 0.3	7, 1
5316	dsrD	Dissimilatory sulfite reductase	3.4 ± 0.2	9, 5
4273	rub	Rubredoxin	2.9 ± 0.1	8, 1
4271	sor	Superoxide reductase	2.8 ± 0.9	7, 15
1642	ppaC	Pyrophosphatase	2.7 ± 0.2	3, 2
3996	ack	Acetate kinase	2.2 ± 0.2	7, 3
223	atpA	ATP Synthase F1 α-subunit	2.1 ± 0.1	1, 8
1072	thcE	Triheme cytochrome c operon membrane protein	2.0 ± 0.1	2, 11

^aMacroarrays were hybridized with cDNA reverse transcribed from RNA extracted from wild-type D. vulgaris grown in WP-LS medium.

^bORF numbers in a preliminary annotation of the genome sequence (http://www.tigr.org).

^cCalculated as indicated in the text. The mean of four determinations and the average deviation from the mean are shown.

^dPosition (row number, column number) of gene on the macroarray depicted in Fig. 3A.