FIG. 2.

Influence of growth at a low temperature on the protein profile of B. subtilis 168. (A and B) Cells were grown in SMM at either 37°C (A) or 15°C (B) to an OD at 578 nm of 1.0. Crude protein extracts were prepared and separated by 2-DE. After being stained with silver nitrate, the gels were scanned with an imaging system and analyzed with the Melanie 3.0 software package. Proteins induced or repressed by growth at the low temperature are marked with arrowheads or circles, respectively. (C) Enlarged sections of gels under both conditions. Representative members of the SigB-dependent general stress regulon are labeled.