FIG. 5.

Time-resolved low-temperature induction of ctc-lacZ in different growth media. The B. subtilis ctc-lacZ fusion strains BSM151 (SigB⁺) (filled circles) and BSM156 (SigB⁻) (open circles) were precultured in SMM at 37°C and used to inoculate cultures in either SMM, LB medium, or DSM. The cultures were then propagated at 16°C. Samples were removed for β-galactosidase assays at the indicated time points. β-Galactosidase activities are expressed in Miller units (MU). Solid lines indicate the growth of the wild-type strain (BSM151); growth of the sigB mutant strain (BSM156) did not differ from that of the wild-type strain under these growth conditions.