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Influence of sigB and glycine betaine on growth at a low temperature. The B. subtilis wild-type strain 168 (squares) and its isogenic sigB mutant BSM29 (circles) were precultured in SMM at 37°C and used to inoculate 25-ml cultures in 100-ml Erlenmeyer flasks with (filled symbols) or without (open symbols) 1 mM glycine betaine. The cultures were then propagated at 15°C.
