FIG. 7.

An RsbV-independent signal transduction cascade controls chill induction of gsiB-gfp. The isogenic set of B. subtilis strains BSM269 (RsbV⁺ SigB⁺), BSM275 (RsbV⁻ SigB⁺), and BSM276 (RsbV⁺ SigB⁻) was precultured in SMM at 37°C and used to inoculate cultures in SMM that were subsequently either exposed to ethanol stress or grown at 16°C. Samples were removed for phase-contrast and fluorescence microscopy from cultures growing exponentially (exp.) at 37 or 16°C (OD at 540 nm, 1.0) or 60 min after cells grown at 37°C had been exposed to 4% ethanol.