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Confocal photomicrographs showing viability within microcolonies in 10-day-old biofilms containing the P. aeruginosa wild type (a), rpoN mutant (b), rpoN mutant with rpoN complemented in trans (c), lasI mutant (d), rhlI mutant (S. Beatson) (e), and an fliM pilA double mutant (f). Scale bar = 50 μm. The results are representative of the results of three experiments.
