FIG. 2.

Growth and MreC expression by the MreC-inducible strain. B. subtilis KSS1571 was cultured in TSB containing 2 mM IPTG overnight at 37°C, diluted 1:100 in fresh medium supplemented with different concentrations of IPTG, and then incubated for 6 h. MO1099, the wild-type MreC control strain, was cultured in TSB without IPTG. Growth was determined by absorbance at 500 nm (B). Then the cells were harvested and lysed, and the cell lysates were electrophoresed and Western blotted. The blots were probed with rabbit anti-MreC antibody (A). In panel B, the mean values from three determinations are shown, and the range of values is indicated.