FIG. 5.

Affinity blotting of selected dockerin-containing fusion proteins performed with recombinant cohesins from ScaA and ScaC. Dockerins from ScaB and the cellulosomal GH9B enzyme were fused to G. stearothermophilus xylanase T6, and the resultant fusion proteins (Xyn-DocB and Xyn-DocGH9, respectively) were expressed in an appropriate E. coli host cell system. The fusion proteins were subjected to SDS-PAGE (Gel), transferred to nitrocellulose membranes (Blots), and probed with the ScaA and ScaC cohesins (CohA5 and CohC3, respectively), as described in the legend to Fig. 4. Lane Std contained prestained protein molecular weight markers.