TABLE 1.

Primers used in this study

Primer	Nucleotide sequencea	Locationb	Comments
PAC-F1	GAAGATGTAATGATAGTTGC	Doc, cipV (scaA)	Probe for XbaI library screening
PAC-R1	GTCTATTGCATTTGGAACTGA	Coh-1, scaB	Probe for XbaI library screening
ACAnF18	CAGCTGCAGCTCCTGAACAGAC	Coh-1, scaB	Sequencing
pAC-F2	CAGGAACATTAGCAGTAGTAG	Coh-2, scaB	Sequencing
pAC-F3	GAAGATTCAGAAACAATGCC	Coh-2, scaB	Sequencing
ACAnF13	GAGGATTACGGACCAATAG	Coh-3, scaB	Sequencing
pAC-Anc-F4	CCACTAGAGGGTGAGATACTTGC	Coh-4, scaB	Sequencing
ACAn R16	GAAGTTTAGTAGACCTTCGC	Coh-4, scaB	Sequencing
ACAn R15	GTCTCTTACTAGAACAGCAT	Doc, scaB	Sequencing
ACAn F15	CCCTGTTGAAGAGAAAGAAG	Doc, scaB	Probe for XR library screening
ACAnR13	CTACTACCATCTACTGGG GC	Coh-1, scaC	Probe for XR library screening
F-AC-Sca1	GGCGTTGAATCTGGAAG	Coh-2, scaC	Sequencing
F-AC-Sca6	CTGTAGCAAGTATAGATGCTGGC	Coh-3, scaC	Sequencing
F-AC-Sca8	CATTCTATTATTCTGGAACTGATG	Coh-3, scaC	Sequencing
R-AC-SCA10	GTTACACCTGGTGTTACACTTCCTG	Linker, scaC	Sequencing
R-AC-SCA9	CACATCCGCACCTGATAACTTAGCC	SLH-1, scaC	Sequencing
R-AC-SCA8	CAAAACTGTTCCATATTCAAGTAA	Downstream of scaC	Sequencing
R-AC-SCA7	CCATTGTTATATAAGACTCATCGCTTGC	Downstream of scaC	Sequencing
R-AC-SCA6	CCAACAACAACTGACGTCTTATC	Downstream of scaC	Sequencing
F-EX-Coh5A	ATATCCATGGATGGTAAAGTAGAGATCATAGAT	Coh-5, cipV (scaA)	Expression
R-EX-Coh5A	AATTCTCGAGCGTTACGTTTCCTACTGTAACAGA	Coh-5, cipV (scaA)	Expression (His tag)
Pac-Anc-CohI-N	TAACCATGGCTCCAACATCTAGTATAG	Coh-1, scaB	Expression
Pac-Anc-CohI-C	TGTCTCGAGACTTGCTTTAATCATATC	Coh-1, scaB	Expression (His tag)
N-AC-Coh3C	CTACCATGGATTTACAGGTTGACATTGGAAGT	Coh-3, scaC	Expression
R-New-Coh3C	CAGCTCGAGACTTGCAATTACCTCAATTTTTCC	Coh-3, scaC	Expression (His tag)
R-AC-Coh3C	CAGGGATCCACTTGCAATTACCTCAATTTTTCC	Coh-3, scaC	Expression (without His tag)
F-9dxyn-docB	TATGGTACCGCCTAAATTTATATATGGTGATGTT	Doc, scaB	Expression, fused to xylanase T6
R-9dxyn-Acdoc	TATGGATCCTTCTTCTTTCTCTTCAACAGGG	Doc, scaB	Expression, fused to xylanase T6
F-9dxyn-docE	ATAGGTACCCCTGCACAATACGTATATGGTGAT	Doc, GH9B	Expression, fused to xylanase T6
R-9dxyn-docE	CAAGGATCCCTTTTGTACCGGAAACTTTGAGAT	Doc, GH9B	Expression, fused to xylanase T6
M13/pUC(-21)	AACAGCTATGACCATGATTACG	Plasmid	Sequencing
M13/pUC(-20)	TGTAAAACGACGGCCAGT	Plasmid	Sequencing
T7	CGCGCGTAATACGACTCACTATAG	Plasmid	Sequencing
SP6	CCAAGCTATTTAGGTGACAC	Plasmid	Sequencing

^aRestriction sites are underlined in primers used for protein expression.

^bDoc, dockerin domain; Coh, cohesin domain; GH9B, dockerin from putative family 9 enzyme.