Figure 1.

HSC and CLP in remission and leukemic BM. Five-color flow cytometric analyses of BM cells in remission (A: case 5b) and in leukemic phase (B: case 5a). BM cells were first gated by the negative expression of lineage-related antigens. (A) The CD34⁺Thy-1⁺CD38^-/lo HSC and CD34⁺Thy-1^-CD10⁺CD38⁺ CLP were sorted from remission BM. (B) In leukemic BM, HSC and CLP were absent, whereas primitive CD34⁺CD38^-/lo leukemic progenitors and CD34⁺CD38⁺ leukemic blasts existed. FSC, forward light scatter.