Table 2.
AML1/ETO mRNA in FACS-purified cells in remission
| Case | AML1/ETO mRNA (105BM cells) | No. of AML1/ETO+ samples/no. of samples tested
|
||||
|---|---|---|---|---|---|---|
| HSC (500 cells) | CLP (500 cells) | Monocytes (500 cells) | B cells (500 cells) | T cells (5,000 cells) | ||
| 5b | + | 0/3 | 0/8 | 4/29 | 4/205 | 0/24 |
| 6b | + | 0/8 | 0/3 | 3/32 | 3/52 | 0/20 |
| 12 | + | 1/12 | 0/23 | 2/11 | 1/82 | 0/25 |
| 13 | + | 1/20 | 0/5 | ND | ND | 0/10 |
| 15 | + | 3/26 | 0/8 | 1/7 | 2/81 | 0/6 |
| 16 | + | 1/20 | 0/30 | 2/9 | 2/37 | 0/13 |
| 17 | + | 0/4 | 0/2 | ND | 1/64 | 0/2 |
| 18 | + | 0/3 | 0/2 | 1/3 | 0/16 | 0/5 |
| 20 | + | 1/9 | 0/7 | 2/17 | 2/140 | 0/6 |
| 22 | + | 1/27 | 1/30 | 3/13 | 3/149 | 0/6 |
| 23 | + | 2/17 | 1/8 | 2/10 | 6/230 | 0/10 |
| 24 | + | 0/11 | 0/4 | 3/16 | 2/41 | 0/9 |
| 25 | + | 0/12 | 0/3 | ND | 0/26 | 0/9 |
| Total | 10/172 (5.8) | 2/133 (1.5) | 23/147 (15.6) | 26/1123 (2.3) | 0/145 (0) | |
Each population was purified by a five-color FACS sorting and pooled either 500 cells (HSC, CLP, monocytes, and B cells) or 5,000 cells (T cells) were analyzed by RT-PCR for AML1/ETO mRNA. Representative data are shown in Fig. 2A. ND, not done.