Table 4.
AML1/ETO mRNA in single-cell-derived myeloid colonies in remission and leukemic phase
| Phase | Case | No. of AML1/ETO+ colonies/no. of colonies tested
|
|||||
|---|---|---|---|---|---|---|---|
| CFU-L | CFU-GM | BFU-E | CFU-Meg | CFU-Mix | Total | ||
| Remission | 5 | 0/0 | 2/58 | 1/30 | 0/28 | 1/28 | 4/144 (2.8) |
| 6 | 0/0 | 2/76 | 1/28 | 0/20 | 0/20 | 3/144 (2.1) | |
| 15 | 0/0 | 2/70 | 0/30 | 1/24 | 0/20 | 3/144 (2.1) | |
| 16 | 0/0 | 3/62 | 0/37 | 0/26 | 1/24 | 4/149 (2.7) | |
| 18 | 0/0 | 4/94 | 2/38 | 0/34 | 0/26 | 6/192 (3.1) | |
| Total | 0/0 | 13/360 (3.6) | 4/163 (2.5) | 1/132 (0.8) | 2/118 (1.7) | 20/773 (2.6) | |
| Leukemic | 2 | 14/14 | 31/48 | 8/17 | 0/0 | 1/2 | 40/67 (59.7) |
| 3 | 4/4 | 3/3 | 2/4 | 1/1 | 0/0 | 6/8 (75.0) | |
| 4 | 6/6 | 7/12 | 1/2 | 0/0 | 0/0 | 8/14 (57.1) | |
| 5 | 3/3 | 1/2 | 1/1 | 0/0 | 0/0 | 2/3 (66.7) | |
| 6 | 12/12 | 24/37 | 2/5 | 0/0 | 0/0 | 26/42 (61.9) | |
| Total | 39/39 (100) | 66/102 (64.7) | 14/29 (48.2) | 1/1 (100) | 1/2 (50) | 82/134 (61.2) | |
FACS-purified 288 single CD34+Thy-1+CD38−/lo cells in remission marrow and 1,440 CD34+Thy-1−CD38−/lo cells in leukemic marrow were cultured in methylcellulose, and all colonies formed were picked on day 14 for RT-PCR analysis. Representative data are shown in Fig. 4.