TABLE 2.
List of oligonucleotides used in this study
| Primer namea | Oligonucleotide sequence (5′ to 3′) | Amplified L. pneumophila Philadelphia-1 gene or region (reference) | Size of amplified region (bp) |
|---|---|---|---|
| F 1 | GCTTACTGAGTTACAACCTGA | Putative activation protein | 1,198 |
| R 1 | GCAATTTGGCAGCGTCAA | ||
| F 5 | GCGCTCAAGACGCTGATTCA | Intergenic region of tRNA valine and acetyltransferase | 562 |
| R 5 | GCACAGATCCACATTAATA | ||
| F 6 | ATCTTGAATTCCTCCACTGTA | Intergenic region of acetyltransferase and putative protein | 872 |
| R 6 | GATTAGCGGATCCTAACAGAA | ||
| F 2 | GCAACCCGGGATGAGTTTGA | Putative phage protein | 1,457 |
| R 2 | GCTGAGCAATTCTTGGTACGA | ||
| F 7 | GCATCGGCACTCTGTATA | 900 bp upstream of transposase AB | 869 |
| R 7 | GCTGATGATATGAGGATA | ||
| F MagA 5134 | CTCTATCGCTAACGCACAAGG | magA | 469 |
| R MagA 5603 | CGTTGAAGTAGTTAGTGAAAG | ||
| F MagA Right | GCCTTCGCCGAGGTGGACAGA | Intergenic region of mercuric reductase and hypothetical protein | 541 |
| R MagA Right | GCTATGGTTAGTTAGACGAGCA | ||
| F 8 | GCTTTATGTGGGATGCCACA | lvrA | 784 |
| R 8 | GCCTGATTTACGCTAAGGA | ||
| F 9 | GCTTATCATCACTTGCCCTTTA | traD | 633 |
| R 9 | GCAGAGATACACCACCAATCCGA | ||
| F 10 | GCAAGATTCCAACCTTCCGCA | Tn5 transposase WO | 894 |
| R 10 | GCGTTGGATCGAGTCTCTCGA | ||
| F 3 | GCGTATTGCGACTCAGGTTA | Putative prophage repressor | 705 |
| R 3 | GCAGTGCAGTTGGCTGAAGTCA | ||
| F 4 | GCCCTAAAGAGGCTGAAATCATA | Mevalonate kinase | 1,013 |
| R 4 | GCTTATGACCTATTAGCTGA | ||
| F VirB4 | GCTTTTATTTCGGGGAGTGC | virB4 (38) | 677 |
| R VirB4 | GTTTCCTCCGGAAAATGGCGG | ||
| F VirD4 | CATAGGCTTTACCGATGATG | virD4 (38) | 414 |
| R VirD4 | AGACTCGGAATAACCTTGGG |
a
F, forward; R, reverse.