TABLE 2.

Purification of TraGΔ2, TraGΔ2K187T, and TrwBΔ1

Protein	Fraction	Purification step	Total protein (mg)	Yield (%)a	Purity (%)b
TraGΔ2	I	Crude extract	645	100	42
	II	Ni-NTA	315	80	69
	III	Phosphocellulose P11	98	31	85
TraGΔ2K187T	I	Crude extract	753	100	30
	II	Ni-NTA	344	94	62
	III	Phosphocellulose P11	107	38	80
TrwBΔ1	I	Crude extract	715	100	44
	II	Ni-NTA	264	81	97
	III	Phosphocellulose P11	261	80	97

^aBased on yield of first fraction of TraGΔ2, TraGΔ2K187T, or TrwBΔ1.

^bLaser densitometric evaluation of Coomassie blue-stained gels.