TABLE 3.
Oligomeric states of TraG, TraGΔ2, and TrwBΔ1
| Protein | Calculated Mr (103) of the monomera |
Mr (103) evaluated by:
|
Deduced oligomeric state | |
|---|---|---|---|---|
| SDS-PAGEb | Gel filtration | |||
| TraG | 70.8 | 71.2 | >1,300c | >18c |
| TraGΔ2 | 59.7 | 59.3 | 50-60 | 1 |
| TrwBΔ1 | 49.57 | 47.3 | 27-33 | 1 |
Calculated from the predicted amino acid sequence.
SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Equivalent to a higher-order oligomer or aggregate.