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. 2003 Aug;185(15):4362–4370. doi: 10.1128/JB.185.15.4362-4370.2003

FIG. 1.

FIG. 1.

Partial purification of β-1,3-glucanase activities from culture filtrate of strain N4-7. (A) Extracellular proteins from strain N4-7 were resolved by phenyl-Sepharose chromatography (solid line), and fractions were assayed for β-1,3-glucanase activity (dotted line). (B) β-1,3-Glucanase activities purified from phenyl-Sepharose activity peaks I and II. Lanes I-1 and I-2, anodic native PAGE of active fractions following separation of peak I by anion exchange chromatography; lane II, cathodic native PAGE of peak II. (C) SDS-PAGE of β-1,3-glucanase-active fractions after chromatographic separations. Lane M, protein molecular mass standards in kilodaltons. All other lanes correspond to those in panel B. Arrows, major bands extracted for protein sequencing.