TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| L. enzymogenes N4-7 | Wild-type biocontrol bacterium | 20 |
| E. coli | ||
| DH5α | Host strain for general cloning; supE44 ΔlacU169φ80lacZΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | |
| HB101 | Host strain for genomic library; F−thi-1 hsdS20 (rB− mB−) supE44 recA13 ara-14 leuB6 proA2 lacY1 rpsL20 (Strr) xyl-5 mtl-1 | |
| TOP10 | Host strain for PBAD expression vectors; F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(araA-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| BL21 (DE3) | Host strain for T7 expression vectors; F−dcm ompT hsdS (rB− mB−) galλ(DE3) | Stratagene |
| Plasmids | ||
| pUC119 | Multicopy cloning vector; Apr | 38 |
| pBluescript SK(+) | Multicopy cloning vector; Apr | Stratagene |
| pLAFR3 | Cosmid cloning vector; Tcr | 34 |
| pBAD/myc-HisA | Multiple cloning site downstream from PBAD promoter; Apr | Invitrogen |
| pET3C | T7 expression vector; BamHI site in reading frame 3 relative to T7 gene 10 initiation codon; Apr | Stratagene |
| pNG2 | pLAFR3 cosmid library clones containing gluA | This study |
| pNG6 | pLAFR3 cosmid library clones containing gluB | This study |
| pNG9 | pLAFR3 cosmid library clones containing gluC | This study |
| pNG21 | 3.2-kb PstI gluA fragment of pNG2 in pUC119; ORF oriented downstream of lac promoter | This study |
| pNG602 | 2.0-kb NotI gluB fragment of pNG6 in pBluescript SK(+); ORF oriented downstream of lac promoter | This study |
| pNG94 | 3.2-kb PstI gluC fragment of pNG9 in pBluescript SK(+); ORF oriented downstream of lac promoter | This study |
| pNG2503 | Coding region of gluA cloned in pET3C | This study |
| pNG6027 | Coding region of gluB cloned in pBAD/myc-HisA | This study |
| pNG9615 | Coding region of gluC cloned in pBAD/myc-HisA | This study |