TABLE 2.
Oligonucleotide primer sequences used for Southern hybridization probes and PCR amplificationa
| Primer | Sequence (5′-3′) | Restriction site |
|---|---|---|
| glu43 | TTCAACCTCGCCCTGCTCAACGCCAGCC AGAACACCGC | |
| glu31 | ACCTACGGCACCATCCACTG | |
| NGAm | CCCGGATCCAGAGCTGGCAGCTGGTCT | BamHI |
| NGAup | GGCGGATCCGAACGCGCGGCTTACTG | BamHI |
| N43m | CCCGGATCCGCCACCCCGGCGCGCTTC AACC | BamHI |
| N43up | GCCGGATCCTCAGCGCAGCGCCCGCAA TGT | BamHI |
| NGCm | CGCCTCGAGGCAGAACTGGCAATTGGT | XhoI |
| NGCup | GCGCTCGAGGAACCCGAGTGTTCAGAT | XhoI |
a
Underlining designates restriction sites. All primers were synthesized by Integrated DNA Technologies, Coralville, Iowa, or Genosys Biotechnologies, Inc., The Woodlands, Tex.