TABLE 3.

Summary of purification of recombinant β-1,3-glucanase-active proteins

Purification step	Total protein (mg)	Total activity (U)	Sp act (U/mg)	Purification (fold)	Yield (%)
GluA
Culture filtrate	420	39	0.09	1	100
Phenyl-Sepharose	1.2	10	8.3	92	26
GluB
Culture filtrate	1,300	53	0.04	1	100
Phenyl-Sepharose	30	20	0.7	17	38
SP Sepharose	5.9	19	3.2	80	36
GluC
Culture filtrate	380	46	0.12	1	100
Phenyl-Sepharose	19	41	2.2	18	89
Q Sepharose	0.13	9	69	575	20