TABLE 4.

Substrate specificity of recombinant β-1,3-glucanase-active proteins

Substrate	Main linkage and properties	Relative activity (%)a of:
		GluAb	GluBc	GluCd
Laminarin	β-1,3; linear, soluble	100 ± 4.1	100 ± 5.3	100 ± 7.9
Curdlan	β-1,3; linear, insoluble	114 ± 7.3	56 ± 2.3	132 ± 0.7
Pachyman	β-1,3; with β-1,6 branches, insoluble	14 ± 0.3	29 ± 1.3	17 ± 1.0
Zymosan A	β-1,3; yeast cell walls, insoluble	47 ± 3.1	148 ± 1.4	62 ± 3.6
CM-cellulosee	β-1,4; linear, soluble	3.9 ± 4.6	3.2 ± 2.5	2.6 ± 1.0
Amylose	α-1,4; linear, soluble	1.6 ± 0.7	1.5 ± 2.5	1.4 ± 2.9

^aActivity ± standard error of triplicate samples relative to laminarin.

^b100% activity = 6.8 U of GluA protein mg⁻¹.

^c100% activity = 2.1 U of GluB protein mg⁻¹.

^d100% activity = 55 U of GluC protein mg⁻¹.

^eCM-cellulose, carboxymethyl cellulose.