TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| Strains | ||
| MC-1 | Coccoid, marine, magnetotactic bacterium | 7 |
| Escherichia coli | ||
| DH5α | supE4 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Clontech |
| BL21(DE3) Codon plus | E. coli B F−ompT hsdSB(rB− mB−) dcm+ Tcrgalλ (DE3) endA Hte[argU ileY leuW Camr] | Stratagene |
| Plasmids | ||
| pGEM-T | PCR cloning vector; Apr | Promega |
| pET15b | His6 tag expression vector; Apr | Novagen |
| pUA1011 | pGEM-T derivative carrying the 678-bp PCR fragment containing the coding region of the MC-1 lexA gene; amplified with primers JC5 and JC6 | This work |
| pUA1013 | pET15b derivative carrying the 678-bp NdeI-BamHI fragment containing the coding region of MC-1 lexA | This work |