Figure 7.

Host proteins in Gag particle: Detergent binding and effect of Env coassembly. (Left) ³⁵S-labeled Gag particles were solubilized with Triton X-114, and protein partitioning was followed during detergent and aqueous phase separation. Unsolubilized material (lane 1) and solubilized material in the aqueous (lane 2) and detergent (lane 3) phases were analyzed by SDS/PAGE together with a sample of starting material (lane 4). Host proteins are indicated. (Right) Gag particles were produced in cells infected with SFV-1/Pr65^gag+Pr80^env and labeled both before and after infection (lane 3) or only after infection (lane 4). The particles were purified by sedimentation in an iodixanol gradient and analyzed by SDS/PAGE. Gag particles produced in cells infected with SFV-C/Pr65^gag (lane 1) and SFV-1/Pr65^gag (lane 2), respectively, were analyzed as controls.