Figure 2.

Relation between adherence of H. pylori (OD₄₉₀) to AGS cells and IL-8 production from AGS cells cocultured with H. pylori clinical isolates (A and B) and oipA knockout mutants (B). H. pylori was added to the cultured cells (bacterium-to-cell ratio of 100:1) for 24 h, and IL-8 in the supernatant was assayed by ELISA. H. pylori was added to the cultured cells (bacterium-to-cell ratio of 500:1) for 90 min, adherent H. pylori and cells were fixed at 4°C for 60 min, and adherence was assayed by ELISA using anti-H. pylori Ab (diluted 1:50) as a first Ab. The OD at 490 nm was used as the index of the number of H. pylori adhering to AGS cells. In B, the beginning of the array shows wild-type strains, and the end of the array shows oipA knockout mutants. The * indicates that the strains have nonfunctional oipA; therefore, the oipA knockout had no effect on IL-8 production.