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IL-8 production from AGS cells cocultured with 16 cag-positive clinical isolates (A) and 6 cag-negative clinical isolates (B) from Japanese and U.S. patients and their oipA or cagE knockout mutants. H. pylori was added to the cultured cells (bacterium-to-cell ratio of 100:1) for 24 h, and IL-8 in the supernatant was assayed in triplicate by ELISA. Error bars indicate mean + SD. The broken line indicates the IL-8 levels from AGS cells without being cocultured with H. pylori (control).
