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Translocation of STE-CyaA fusion proteins. Wild-type S. typhimurium or strains carrying mutations in SPI2 TTSS (ssaT) or SPI1 TTSS (ΔsspC) expressing various CyaA fusions were used to infect RAW264.7 cells as described in Fig. 3, assaying translocation by SPI2 (A) or SPI1 (B) TTSS. SspH1208, SspH2214, SlrP191, SseI206, SseJ147 and SspA158 and LacZ21CyaA fusions were analyzed.
