Western blots of the supernatant and total cell fractions from different constructs in A. tumefaciens strain A348. Cells were grown at 28°C under vir gene-inducing conditions, separated into supernatant and total cell fractions (noted on the top). The proteins were detected by using specific antibodies (noted on the left). Lane 1, A348; lane 2, A348 (pUFR047); lane 3, A348 (ptac-ros/pUFR047); and lane 4, A348 (ptac-CAT/pUFR047). Total cell proteins, TCP; and supernatant proteins, SP.