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. 2000 Jun 13;97(13):7545–7550. doi: 10.1073/pnas.120156997

Figure 5.

Figure 5

Western blots of A. tumefaciens cells containing pRSF1010 (pML122ΔGm) and Osa (ptac-osa/pUFR047). (A) Supernatant fractions were harvested from cells grown under noninducing conditions, and proteins were detected with VirD2 and VirE2 antibodies. Lane 1, A136 (ptac-virE1 + E2/pUFR047 + pML122ΔGm); and lane 2, A136 (ptac-virE1 + E2/pUFR047). (B) Western blot analysis of supernatant and total cell fractions of A. tumefaciens strains carrying Osa. Fractions (noted on the top) were collected from cells grown under inducing conditions, and proteins were detected with specific antibodies (noted on the left). Lane 1, A348 (pUFR047); and lane 2, A348 (ptac-osa/pUFR047).