Fig. 3.

Promoter nucleosome mobilization requires the TATA box. MNase ladders were generated from nuclei of cells carrying either wild-type, HS2ε,or HS2ε-globin genes with mutations in promoter GATA, CACC, or TATA motifs. (A) Hybridization was performed with the N1– probe. imagequant data and excel and sigmaplot software were used to produce scans of the most highly digested lane for each of the clones. (B) Blots were stripped and rehybridized with the N2–/N3– probe. For scans, see Fig. 7.