Fig. 4.

Promoter H3 and H4 acetylation requires the transcription activators and the TATA box. Representative ChIPs performed on nuclei of cells carrying either wild-type HS2ε, or HS2ε-globin genes with mutations in promoter GATA, CACC, or TATA motifs using antibodies to diacetylated H3 (A) or hyperacetylated H4 (B). The results of three experiments with N1– (black bars, ± SEM) or ampicillin primers (gray bars, ± SEM) are depicted graphically. Band intensities for immunoprecipitate (Ab) and control (no, no antibody) samples are expressed relative to the signal from 0.5% of input (In) DNA to correct for amount of chromatin and minichromosome copy number and are normalized to wild-type HS2ε.