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Decahistidine-tagged Yvc1p expressed from a plasmid retains the ≈400-pS unitary conductance and its mechanosensitivity. Vacuole bathed in a solution containing 10-4 M Ca2+ and held at -20 mV. Repeated pressure pulses of 50 mmHg yielded repeated channel responses from the vacuole. A gap of 52.3 sec separated episodes 3 and 4, showing no long-term effects of repeated activations.
