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. 2003 May 22;100(12):7111–7116. doi: 10.1073/pnas.1232352100

Fig. 2.

Fig. 2.

PGC-1α coactivates MEF2s on the PGC-1α promoter. (A) MEF2C and MEF2D activate the mouse PGC-1α promoter. C2C12 cells were cotransfected with expression plasmids for MEF2C, MEF2D, NFAT, and PGC-1α together with reporter gene plasmids containing 2 kb of the mouse PGC-1α promoter. After 48 h, cells were harvested and luciferase activity was determined. (B) MEF2 activity is increased by CnA. C2C12 cells were cotransfected with expression plasmids for MEF2C, MEF2D, NFAT, CnA, and PGC-1α together with reporter gene plasmids containing 6 kb of the mouse PGC-1α promoter. After 48 h, cells were harvested and luciferase activity was determined. The values represent the average of three independent experiments, and bars represent SD. *, P < 0.5 between different treatments compared with the untreated control.