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. 2003 Jun 2;100(12):7135–7140. doi: 10.1073/pnas.1231422100

Fig. 2.

Fig. 2.

Efficiency of Raldh2-/- embryonic rescue with natural and synthetic retinoids. E10.5 wild-type (WT) and Raldh2-/- embryos are shown from pregnant mice that were untreated (A and B) or treated on E7.25–8.25 with 2.5 mg/kg all-trans-RA (atRA, C and D), 2.5 mg/kg 9-cis-RA (9cRA, E and F), 10 mg/kg 9-cis-RA (G and H), 0.5 mg/kg RAR-specific SHet100 (I and J), and 2.5 mg/kg RXR-specific OHet72 (K and L). Also, the extent of RARE-lacZ transgene expression is compared for each embryo as an indicator of where retinoid signaling is occurring. It can be seen that treatment with 2.5 mg/kg all-trans-RA, 10 mg/kg 9-cis-RA, and 0.5 mg/kg SHet100 results in a very similar rescued phenotype. However, it should be stressed that, whereas rescued Raldh2-/- embryos appear grossly normal, forelimb outgrowth is severely retarded (see D, H, and J), and other developmental abnormalities such as those reported for rhombomere segmentation (43) and cardiac development (44) may still exist.