Figure 3.

Inhibition of MEF2 function decreases the number of multipotent and unipotent precursor cells. Control cultures (clone 2–1) and MEF2 dominant negative cultures (clone 2–7) were treated with retinoic acid for 3.0 or 3.5 days. (A and C) Cells incubated with anti-nestin to label multipotent precursor cells (A) or anti-Hu to label unipotent precursor cells (C). Labeled cells were visualized with peroxidase. (B and D) The number of nestin-positive cells (B) and Hu-positive cells (D) in 40 randomly selected fields was scored in a blinded fashion. Values are mean ± SD from at least three independent experiments (*, P < 0.02 by Student's t test).