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HCV E1E2 gp expression. Transfected 293-T cells were tested for expression of HCV E1 and E2 gps. Cells transfected with pNL4.3.Luc.R-E- and pE1E2 were tested for cell surface and total expression of E1 (mAb H111), E2 (mAbs CBH5 or H52), and PDI (SPA-891). Cells were fixed and analyzed for antigen expression with (B) or without (A) permeabilization. Regions were established based on irrelevant isotype-matched IgG staining of the cells.
