Table 1. Cell phenotype and susceptibility to HIV pseudotype infection.
| Infectivity of pseudotypes expressing env gps*
|
Cell-surface expression†
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cell line | Cell type | No env | SF162 | VSV G | H E1E2 | Con1 E1E2 | CD81 | LDLR | SR-B1 |
| Hos.CD4.R5 | Human osteosarcoma | - | ++ | +++ | - | - | +++ | + | + |
| Huh-7.5 | Human hepatoma | - | - | +++ | ++ | ++ | ++ | + | + |
| Huh-7 | Human hepatoma | - | - | +++ | ++ | ++ | ++ | + | + |
| HepG2 | Human hepatoma | - | - | +++ | - | - | - | + | + |
| PLC/PR5 | Human hepatoma | - | - | ++ | + | + | ++ | + | - |
| THLE | Human liver epithelial | - | - | +++ | - | - | +++ | - | - |
| Hela | Human epithelial | - | - | ++ | - | - | +++ | - | + |
| SW13 | Human colorectal adenocarcinoma | - | - | +++ | - | - | +++ | + | + |
| U937 | Human monocytoid | - | - | + | - | - | - | - | - |
| U937-humCD81 | Human monocytoid | - | - | + | - | - | +++ | - | - |
| THP | Human monocytoid | - | - | ++ | - | - | +++ | - | ++ |
| THP-DC-SIGN | Human monocytoid | - | - | ++ | - | - | +++ | - | ++ |
| RBL | Rat basophil | - | - | ++ | - | - | - | - | - |
| RBL-humCD81 | Rat basophil | - | - | ++ | - | - | +++ | - | - |
Virus pseudotypes with no env, H E1E2, Con1 E1E2, and SF162 were tested for infection of the various cell lines at 5 and 1 ng per well (data not shown) and VSV G at 0.1 ng per well
*
Infectivity is measured as RLU where: -, <300; +, 10,000-20,000; ++, >20,000-100,000; and +++, >100,000
†
Cell-surface expression of antigens, where an irrelevant isotype-matched IgG gave mean fluorescence intensity (MFI) in the range of 4-6 and specific mAbs gave staining intensities of +, 10-30; ++, >30-100; and +++, >100