Fig. 4.

Lipoprotein cholesterol profiles and morphology. Parental and offspring mice were fed a low-fat chow diet without or with probucol [0.5% (wt/wt) supplementation]. (A) Representative chromatograms of the TC (mg/dl of plasma) from size-fractionated (Superose 6-FPLC) plasma lipoproteins from 6-week-old untreated dKO (•) or probucol-treated dKO (○) mice are shown with the approximate elution positions of VLDL, intermediate-density lipoproteins (IDL)/LDL, and HDL. (Inset) Expanded HDL region. (B) Electron micrographs of negatively stained lipoproteins from pooled FPLC fractions. Abnormal morphologies are indicated by arrows (lamellar/vesicular), arrowheads (stacked discoidal), and asterisks (aggregated). (Scale bar = 60 nm.)